Identification of spring viraemia of carp virus (SVCV) by combined RT-PCR and nested PCR
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چکیده
منابع مشابه
Identification of spring viraemia of carp virus (SVCV) by combined RT-PCR and nested PCR.
A combination of single-tube reverse transcription (RT)-PCR and nested PCR was used to identify spring viraemia of carp rhabdovirus (SVCV) in infected cell cultures and fish tissues. Two pairs of specific primers (external and internal) were selected from the glycoprotein gene sequence. A specific product of 470 bp was amplified from RNA derived from 34 SVCV isolates including the reference str...
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Monoclonal antibodies (MAbs) against spring viraemia of carp virus (SVCV), a severe disease in cyprinid fish, were prepared. Nine MAbs were characterised using Western blotting (WB) where all reacted with glycoprotein G, except for MAb 2E1, which showed no reactivity in WB. All nine MAbs showed specificity in an immunoperoxidase test. In ELISA assays, their titres ranged between 1:32 000 and 1:...
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متن کاملSpring Viraemia of Carp
The aetiological agent of SVC is spring viraemia of carp virus (SVCV), a member of the virus family Rhabdoviridae, and tentatively placed in the genus Vesiculovirus (21). The virus genome is a nonsegmented, negative-sense, single strand of RNA. The genome contains 11,019 nucleotides encoding five proteins in the following order: a nucleoprotein (N), a phosphoprotein (P), a matrix protein (M), a...
متن کاملمقایسه چهار کیت استخراج RNA تجاری به منظور تشخیص ویروس ویرمی بهاره کپور معمولی (Spring Viraemia of Carp Virus)SVCV
ویرمی بهاره کپور (SVC)، توسط ویروسی باRNA تک رشته ای منفی از خانواده رابدوویریده ایجاد می شود. ویروس SVCV عامل اصلی بیماری مسری ویرمی بهاره کپور در سرتاسر جهان بخصوص در ماهی کپور معمولی پراکنده است و در دیگر خانواده های ماهیان شامل اردک ماهیان ، کپور ماهیان دندانه دار ، خورشید ماهیان ، گربه ماهیان و آزاد ماهیان نیز سبب بیماری می گردد. در این مطالعه به منظور دستیابی به روشی مناسب برای استخر...
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ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 2003
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao055229